| Gene Symbol | PAFAH1B1 |
|---|---|
| Entrez Gene | 5048 |
| Alt Symbol | LIS1, LIS2, MDCR, MDS, PAFAH |
| Species | Human |
| Gene Type | protein-coding |
| Description | platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) |
| Other Description | lissencephaly 1 protein|platelet-activating factor acetylhydrolase IB subunit alpha|platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)|platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa) |
| Swissprots | Q8WZ89 B2R7Q7 Q8WZ88 P43034 |
| Accessions | AAC51111 EAW90536 P43034 AF208835 AF208836 AF208837 AAL34972 AF208838 AAL34973 AF400434 AAK92483 AK293918 BAG57299 AK303015 BAG64145 AK313078 BAG35904 BC064638 AAH64638 BX538346 CAD98141 L13385 AAA02880 L13386 AAA02881 L13387 AAA02882 L13388 L25107 S81396 XM_011523901 XP_011522203 XM_011523902 XP_011522204 XM_011523903 XP_011522205 XM_011523904 XP_011522206 NM_000430 NP_000421 |
| Function | Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration i |
| Subcellular Location | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle {ECO:0000255|HAMAP- Rule:MF_03141}. Nucleus membrane {ECO:0000255|HAMAP- Rule:MF_03141}. Note=Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. {ECO:0000250}. |
| Tissue Specificity | Fairly ubiquitous expression in both the frontal and occipital areas of the brain. |
| Top Pathways | Ether lipid metabolism |
LIS1 CRISPR/Cas9 KO Plasmid (h) - sc-402193 from Santa Cruz Biotechnology
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LIS1 HDR Plasmid (h) - sc-402193-HDR from Santa Cruz Biotechnology
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LIS1 Double Nickase Plasmid (h) - sc-402193-NIC from Santa Cruz Biotechnology
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LIS1 Double Nickase Plasmid (h2) - sc-402193-NIC-2 from Santa Cruz Biotechnology
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LIS1 CRISPR Activation Plasmid (h) - sc-402193-ACT from Santa Cruz Biotechnology
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LIS1 CRISPR Activation Plasmid (h2) - sc-402193-ACT-2 from Santa Cruz Biotechnology
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LIS1 Lentiviral Activation Particles (h) - sc-402193-LAC from Santa Cruz Biotechnology
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LIS1 Lentiviral Activation Particles (h2) - sc-402193-LAC-2 from Santa Cruz Biotechnology
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LIS1 siRNA (h) - sc-35814 from Santa Cruz Biotechnology
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LIS1 shRNA Plasmid (h) - sc-35814-SH from Santa Cruz Biotechnology
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LIS1 shRNA (h) Lentiviral Particles - sc-35814-V from Santa Cruz Biotechnology
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LIS1 RNAi - H00005048-R01 from Novus Biologicals
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MISSION® esiRNA - EHU105321 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN16271 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16271 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR14197 from Sigma-Aldrich
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PAFAH1B1 siRNA (Human) - CRH3354 from Cohesion Biosciences
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shRNA set against Human PAFAH1B1 (NM_000430.3) - SHH370000 from Creative biogene
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shRNA set against Human PAFAH1B1(NM_000430.3) - SHR077928 from Creative biogene
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PAFAH1B1 shRNA Plasmid (Human) - CHH3354 from Cohesion Biosciences
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PAFAH1B1 miRNA 3'UTR clone - MiUTR1H-07462 from Creative biogene
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