NT5C3A

Gene Symbol	NT5C3A
Entrez Gene	51251
Alt Symbol	NT5C3, P5'N-1, P5N-1, PN-I, POMP, PSN1, UMPH, UMPH1, cN-III, hUMP1, p36
Species	Human
Gene Type	protein-coding
Description	5'-nucleotidase, cytosolic IIIA
Other Description	5'-nucleotidase, cytosolic III\|cytosolic 5'-nucleotidase 3\|cytosolic 5'-nucleotidase 3A\|lupin\|pyrimidine 5'-nucleotidase 1\|uridine 5'-monophosphate hydrolase 1
Swissprots	Q9UC45 B8ZZC4 B2RAA5 Q9UC43 Q9P0P5 Q7L3G6 Q6IPZ1 A8K253 Q6NXS6 Q9UC44 Q9UC42 Q9H0P0
Accessions	EAL24463 EAW94007 EAW94008 EAW94009 EAW94010 EAW94011 Q9H0P0 AA482595 AF151067 AAF36153 AF312735 AAG33630 AK290118 BAF82807 AK314109 BAG36802 AL136716 CAB66650 AM393138 CAL38016 AM393316 CAL38194 AM393481 CAL38358 AW339593 BC013292 AAH13292 BC015856 AAH15856 BC066914 AAH66914 BC071652 AAH71652 BF790086 CB127255 CB127877 CB137440 CR533518 CAG38549 DA975244 DB243020 DB346026 DQ892843 ABM83769 EU176737 ABW03538 N98231 XM_011515409 XP_011513711 NM_001002009 NP_001002009 NM_001002010 NP_001002010 NM_001166118 NP_001159590 NM_016489 NP_057573
Function	Can act both as nucleotidase and as phosphotransferase.
Subcellular Location	Cytoplasm {ECO:0000305}.
Tissue Specificity	Isoform 1 and isoform 3 are expressed in reticulocytes and lymphocytes. Isoform 4 is expressed only in reticulocytes. {ECO:0000269\|PubMed:11369620, ECO:0000269\|PubMed:15238149}.
Top Pathways	Purine metabolism, Nicotinate and nicotinamide metabolism, Pyrimidine metabolism

NT5C3 (E-8) - sc-390782 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

NT5C3 (E-8): sc-390782. Western blot analysis of NT5C3 expression in Jurkat (A) and Hep G2 (B) whole cell lysates.

NT5C3 (H-109) - sc-366321 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

NT5C3 (H-109): sc-366321. Western blot analysis of NT5C3 expression in Jurkat whole cell lysate.

NT5C3 (K-14) - sc-104473 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NT5C3 (S-13) - sc-104474 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

NT5C3 (T-25) - sc-133850 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	50 µg/0.5 ml
Host	Rabbit
Clonality	Polyclonal

NT5C3 (T-25): sc-133850. Western blot analysis of NT5C3 expression in Hep G2 whole cell lysate.

Anti-NT5C3 antibody - ab137224 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3 antibody (ab137224) at 1/500 dilution + 293 cell lysate at 30 µg

Anti-NT5C3 antibody - ab197314 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-NT5C3 antibody (ab197314) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : HeLa cell lysate

Anti-NT5C3 antibody - ab67133 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-NT5C3 antibody (ab67133) at 1/500 dilutionLane 1 : NT5C3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg/ml per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-NT5C3A Antibody (aa1-297) - LS-C292551 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3A Antibody (aa1-297, Biotin) - LS-C301138 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3A Antibody (aa1-297, FITC) - LS-C304485 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3A Antibody (aa185-234) - LS-C29706 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3A Antibody (aa36-85) - LS-C200288 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of the lysates from 293 cells using NT5C3 antibody.

Anti-NT5C3A Antibody - LS-C292552 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of recombinant NT5C3A / p36.

Anti-NT5C3A Antibody (aa52-336) - LS-C314974 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3A Antibody (aa11-60) - LS-C119257 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human brain tissue, using NT5C3 Antibody. The picture on the right is treated with the synthesized peptide.

NT5C3 Antibody - NBP2-38124 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NT5C3 Antibody [NBP2-38124] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative Control. Lane 3: Overexpression lysate

NT5C3 Antibody - NBP1-85525 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NT5C3 Antibody [NBP1-85525] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

NT5C3 Antibody - H00051251-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: NT5C3 Antibody [H00051251-B01P] - Analysis of NT5C3 expression in transfected 293T cell line by NT5C3 polyclonal antibody. Lane 1: NT5C3 transfected lysate(31.57 KDa). Lane 2: Non-transfected lysate.

Anti-NT5C3 (181-195) antibody produced in rabbit - N1914 from Sigma-Aldrich

Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-NT5C3 (181-195): <B>Cat. No. N1914</B>: Immunofluorescence of HUVEC cells using NT5C3 (181-195) (RB),<B>Cat. No. N1914</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-NT5C3 antibody produced in rabbit - HPA029058 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-NT5C3: <B>Cat. No. HPA029058</B>: Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Anti-NT5C3 antibody produced in rabbit - SAB2101657 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-NT5C3: <B>Cat. No.SAB2101657</B>: NT5C3 in HepG2 cell lysate with NT5C3 antibody at 1.0 μg/mL.

Anti-NT5C3, N-Terminal antibody produced in rabbit - SAB4502112 from Sigma-Aldrich

Prices	$335.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-NT5C3, N-Terminal: <B>Cat. No. SAB4502112</B>: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-NT5C3, N-Terminal antibody. Cells on the left were treated with the Anti-NT5C3, N-Terminal antibody. Cells on the right (negative control) were treated with both Anti-NT5C3, N-Terminal antibody and the synthesized immunogen peptide.

NT5C3 antibody - middle region (ARP32185_P050) - ARP32185_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

NT5C3 antibody - GTX87639 from GeneTex

Prices	$319.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

Anti-NT5C3 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX87639

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