DCP1B

Gene Symbol DCP1B
Entrez Gene 196513
Alt Symbol DCP1, hDcp1b
Species Human
Gene Type protein-coding
Description decapping mRNA 1B
Other Description DCP1 decapping enzyme homolog B|decapping enzyme hDcp1b|mRNA-decapping enzyme 1B
Swissprots Q96MZ8 Q86XH9 B4DRD1 Q96BP8 Q8IZD4
Accessions EAW88915 EAW88916 Q8IZD4 AK056200 BAB71118 AK299203 BAG61243 AK301109 BAG62708 AK310475 AW204088 AY146652 AAN62764 BC015368 AAH15368 BC043437 AAH43437 XM_011520927 XP_011519229 XM_011520928 XP_011519230 NM_152640 NP_689853
Function May play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. May remove the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15067023}. Nucleus {ECO:0000250}.
Top Pathways RNA degradation

DCP1B (D2P9W) Rabbit mAb - 13233 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2P9W
Western blot analysis of extracts from Hep G2 and MCF7 cells using DCP1B (D2P9W) Rabbit mAb.

Anti-DCP1B antibody - ab103254 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DCP1B antibody (ab103254) at 1/100 dilution + Ramos cell lysate at 35 µg

Anti-DCP1B antibody - ab129493 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of DCP1B by Western Blot of Immunprecipitate. anti-DCP1B antibody at 0.4µg/ml staining DCP1B in HeLa whole cell lysate immunoprecipitated using ab129493 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-DCP1B antibody - ab129514 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of DCP1B by Western blot of immunoprecipitate  ab129514, at 6 µg/ml, immunoprecipitating DCP1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).  A different DCP1B antibody was used at 0.4 µg/ml for blotting the immunoprecipitated DCP1B.  Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-DCP1B Antibody (aa261-290, AP) - LS-C255931 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa261-290, APC) - LS-C255934 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa218-267) - LS-C82636 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-DCP1B Antibody (aa261-290, PE) - LS-C255938 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa261-290, Azide-free, HRP) - LS-C255937 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa261-290, Biotin) - LS-C255935 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa261-290, FITC) - LS-C255936 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa250-300) - LS-C290299 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa200-250) - LS-C290300 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa268-298) - LS-C320759 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B Antibody (aa261-290) - LS-C167298 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of DCP1B Antibody in Ramos cell line lysates (35 ug/lane). DCP1B (arrow) was detected using the purified antibody.

DCP1B Antibody - NBP1-82018 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DCP1B Antibody [NBP1-82018] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp

DCP1B Antibody - NBP1-79422 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: DCP1B Antibody [NBP1-79422] - 721_B cell lysate, concentration 0.2-1 ug/ml.

DCP1B Antibody - NBP2-04009 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DCP1B Antibody - NBP2-04008 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: DCP1B Antibody [NBP2-04008] - Samples:  Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DCP1B antibody NBP2-04008 used for IP at 6 ug/mg lysate.  DCP1B was also immunoprecipitated by rabbit anti-DCP1B antibody NBP2-04009, which recognizes a downstream epitope.  For blotting immunoprecipitated DCP1B, NBP2-04008 was used at 0.4 ug/ml.  Detection: Chemiluminescence with an exposure time of 30 seconds.

DCP1B Antibody - H00196513-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: DCP1B Antibody [H00196513-B01P] - Analysis of DCP1B expression in transfected 293T cell line by DCP1B polyclonal antibody.  Lane 1: DCP1B transfected lysate(67.80 KDa). Lane 2: Non-transfected lysate.

Anti-DCP1B antibody produced in rabbit - HPA039709 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DCP1B: <B>Cat. No. HPA039709</B>: Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-DCP1B (276-290) antibody produced in rabbit - SAB1103531 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DCP1B was separated on SDS-PAGE, blotted with Anti-DCP1B (276-290) (<B>Cat. No.SAB1103531</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + DCP1B immunizing peptide (human, 276-290)

Anti-DCP1B antibody produced in rabbit - SAB2100541 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DCP1B (41-55) antibody produced in rabbit - SAB1103530 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG<SUP>®</SUP>-DCP1B fusion protein was separated on SDS-PAGE, blotted with Anti-DCP1B (41-55) (<B>Cat. No.SAB1103530</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + DCP1B immunizing peptide (human, 41-55)<BR/>3. Negative control (only secondary antibody)<BR/>4. Anti-FLAG<SUP>®</SUP> antibody (<B>Cat. No. F7425</B>), working concentration 2 ug/ml.

DCP1B antibody - N-terminal region (ARP50809_P050) - ARP50809_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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