RAD17

Gene Symbol RAD17
Entrez Gene 5884
Alt Symbol CCYC, HRAD17, R24L, RAD17SP, RAD24
Species Human
Gene Type protein-coding
Description RAD17 homolog (S. pombe)
Other Description RAD1 homolog|RF-C activator 1 homolog|RF-C/activator 1 homolog|Rad17-like protein|cell cycle checkpoint protein (RAD17)|cell cycle checkpoint protein RAD17
Swissprots Q7Z3S4 Q9UNR7 O75714 Q9UPF5 O75943 D3DWA5 A8K8X2 Q9UNK7 Q9UNR8
Accessions AAD42176 AAT09763 CAB46364 EAW51279 EAW51280 EAW51281 EAW51282 EAW51283 EAW51284 EAW51285 EAW51286 EAW51287 EAW51288 EAW51289 O75943 AF017748 AAD01620 AF076838 AAC95520 AF085736 AAC36334 AF098533 AAC97950 AF098534 AAC97951 AF112263 AAD38878 AF126424 AAD17334 AJ001642 CAA04894 AJ004977 CAA06251 AK292487 BAF85176 AL122068 CAB59244 AM393045 CAL37923 BC018110 BC032304 AAH32304 BX537441 CAD97683 DQ891608 ABM82534 DQ894801 ABM85727 XM_005276774 XP_005276831 XM_011546954 XP_011545256 XM_011546955 XP_011545257 NM_001278622 NP_001265551 NM_002873 NP_002864 NM_133338 NP_579916 NM_133339 NP_579917 NM_133340 NP_579918 NM_133341 NP_579919 NM_133342 NP_579920 NM_133343 NP_579921 NM_133344 NP_579922
Function Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. {ECO:0000269|PubMed:10208430, ECO:0000269|PubMed:11418864, ECO:0000269|PubMed:11687627, ECO:0000269|PubMed:11799063, ECO:0000269|PubMed:12578958, ECO:0000269|PubMed:12672690, ECO:0000269|PubMed:14500819, ECO:0000269|PubMed:14624239, ECO:0000269|PubMed:15235112, ECO:0000269|PubMed:15538388, ECO:0000269|PubMed:21659603}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10232579, ECO:0000269|PubMed:10593953, ECO:0000269|PubMed:11799063, ECO:0000269|PubMed:12400013}. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
Tissue Specificity Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors). {ECO:0000269|PubMed:10208430, ECO:0000269|PubMed:10232579, ECO:0000269|PubMed:10480350, ECO:0000269|PubMed:11602352, ECO:0000269|PubMed:9660800}.

Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb - 13404 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6K9P
Western blot analysis of A549 cell extracts, untreated (-) or UV-treated (100 mJ, 18 hr recovery; +), using Phospho-Rad17 (Ser635) (D6K9P) Rabbit mAb (upper) or Rad17 (D3G6) Rabbit mAb #8561 (lower).

Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb - 6981 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5H5
Western blot analysis of extracts from HeLa cells, untreated or treated with CIP and λ phosphatase, using Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb (upper) or Rad17 (D3G6) Rabbit mAb #8561 (lower).

Rad17 (D3G6) Rabbit mAb - 8561 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3G6
Western blot analysis of extracts from various cell lines using Rad17 (D3G6) Rabbit mAb.

Phospho-Rad17 (Ser645) Antibody - 3421 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rad17 Antibody - 3422 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rad17 (C-19) - sc-12416 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad17 (C-19): sc-12416. Western blot analysis of Rad17 expression in non-transfected: sc-117752 (A) and human Rad17 transfected: sc-114889 (B) 293T whole cell lysates.

Rad17 (H-3) - sc-17761 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad17 (H-3): sc-17761 Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis cancer showing nuclear staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Rad17 (H-300) - sc-5613 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rad17 (H-300): sc-5613. Western blot analysis of Rad17 expression in Jurkat (A), HeLa (B) and K-562 (C) nuclear extracts.

Rad17 (P-19) - sc-9377 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rad17 (P-19): sc-9377. Western blot analysis of Rad17 expression in Jurkat nuclear extract.

p-Rad17 (Ser 656) - sc-32843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

p-Rad17 (Ser 646) - sc-32842 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Rad17 (phospho S635) antibody - ab26335 from Abcam

Prices $242.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This antibody gave a positive result in ELISA against the immunizing peptide (ab28625). It gave a negative result in ELISA against the non-modified equivalent peptide (ab28626). This indicates that it is specific for the modified peptide.

Anti-Rad17 (phospho S645) antibody - ab3620 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-Rad17 (phospho S645) antibody - ab63442 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad17 (phospho S645) antibody (ab63442) at 1/500 dilutionLane 1 : HeLa cell extracts (5-30µg total protein) treated with UV (15mins)Lane 2 : HeLa cell extracts (5-30µg total protein) treated with UV (15mins) with Immunising peptide at 10 µg

Anti-Rad17 (phospho S645) antibody [EP1519Y] - ab75779 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1519Y
All lanes : Anti-Rad17 (phospho S645) antibody [EP1519Y] (ab75779) at 1/5000 dilutionLane 1 : HeLa cell lysates, untreatedLane 2 : HeLa cell lysates, treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Rad17 (phospho S646) antibody - ab183893 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad17 (phospho S646) antibody (ab183893) at 1/250 dilutionLane 1 : HeLa whole cell lysate/extract (untreated)Lane 2 : HeLa whole cell lysate/extract (30 µM cisplatin treatment for 24 hours)Lysates/proteins at 30 µg per lane.

Anti-Rad17 (phospho S656) antibody [EPR3145] - ab76012 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3145
All lanes : Anti-Rad17 (phospho S656) antibody [EPR3145] (ab76012) at 1/10000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate treated with Calyculin ALysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Rad17 antibody - ab151513 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad17 antibody (ab151513) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Rad17 antibody - ab186837 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad17 antibody (ab186837) at 1/500 dilutionLane 1 : SW480 cell extractsLane 2 : BT474 cell extractsLane 3 : K562 cell extractsLane 4 : 22RV-1 cell extractsLane 5 : Raji cell extracts

Anti-Rad17 antibody - ab63509 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/50 ab63509, staining Rad17 on human breast carcinoma tissue, in the absence (left) and presence (right) of the immunizing peptide

Anti-Rad17 antibody - ab69817 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad17 antibody (ab69817) at 0.5 µg/mlLane 1 : Rat kidney lysateLane 2 : 3T3 cell lysateLane 3 : Jurkat cell lysateLane 4 : Jurkat cell lysate

Anti-Rad17 antibody - ab195963 from Abcam

Prices $370.00
Sizes 50 µl
Host Goat
Clonality Polyclonal
All lanes : Anti-Rad17 antibody (ab195963) at 0.1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : HeLa whole cell lysate treated with 10 Gy ionizing radiationLysates/proteins at 30 µg per lane.developed using the ECL technique

Anti-Rad17 antibody - ab2847 from Abcam

Prices $370.00
Sizes 50 µl
Host Goat
Clonality Polyclonal

Anti-Rad17 antibody - ab56043 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Rad17 antibody (ab56043) at 1.25 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Rad17 antibody - ab137402 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Rad17 antibody (ab137402) at 1/500 dilution + 293T nuclear extract at 30 µg
Search more