KSR2

Gene Symbol KSR2
Entrez Gene 283455
Alt Symbol -
Species Human
Gene Type protein-coding
Description kinase suppressor of ras 2
Other Description kinase suppressor of Ras 2
Swissprots A0PJT2 Q6VAB6 Q8N775 Q3B828
Accessions CAH68720 EAW98111 EAW98112 Q6VAB6 AK098831 BAC05426 AY345972 AAQ24226 BC107106 AAI07107 BC107107 AAI07108 BC127603 AAI27604 BX648958 DW009880 DW009881 XM_011538224 XP_011536526 XM_011538225 XP_011536527 XM_011538226 XP_011536528 XM_011538227 XP_011536529 XM_011538228 XP_011536530 XM_011538229 XP_011536531 XM_011538230 XP_011536532 XR_944522 NM_173598 NP_775869
Function Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8- mediated signaling. Acts as a negative regulator of MAP3K3- mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. {ECO:0000269|PubMed:12975377, ECO:0000269|PubMed:16039990, ECO:0000269|PubMed:21441910}.
Subcellular Location Cytoplasm {ECO:0000250}. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Tissue Specificity Mainly expressed in brain and kidney. {ECO:0000269|PubMed:12975377}.

Ksr-2 (H-80) - sc-98448 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Ksr-2 (I-19) - sc-50034 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ksr-2 (I-19): sc-50034. Western blot analysis of Ksr-2 expression in A431 (A) and Jurkat (B) whole cell lysates.

Ksr-2 (P-15) - sc-50036 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ksr-2 (P-15): sc-50036. Western blot analysis of Ksr-2 expression in HeLa whole cell lysate.

Ksr-2 (K75) - sc-100421 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Ksr-2 (K75): sc-100421. Western blot analysis of Ksr-2 expression in A-431 whole cell lysate.

Anti-KSR2 antibody - ab72753 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilutionLane 1 : 293 cell extractLane 2 : 293 cell extract with immunising peptide at 10 µgLysates/proteins at 10 µg per lane.

Anti-KSR2 antibody - ab170442 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Flow cytometry analysis of MCF-7 cells (green) compared to a negative control cells (blue) using ab170442 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-KSR2 antibody - ab58018 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HEK293 cells stained with ab58018 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58018, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-KSR2 antibody - ab173377 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-KSR2 antibody (ab173377) at 1 µg/mlLane 1 : KSR2-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-KSR2 antibody - C-terminal - ab172977 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-KSR2 antibody - C-terminal (ab172977) at 1/100 dilution + Human normal uterus tissue lysate at 35 µg

Anti-KSR2 Antibody (aa603-638) - LS-C165650 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
KSR2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-KSR2 Antibody (Ile694) IHC-plus™ - LS-B9334 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-KSR2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-KSR2 Antibody (C-Terminus, AP) - LS-C236990 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (C-Terminus, APC) - LS-C236989 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (C-Terminus, FITC) - LS-C236992 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (C-Terminus, Biotin) - LS-C236991 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (aa808-836, AP) - LS-C236995 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (aa808-836, APC) - LS-C236994 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (C-Terminus, PE) - LS-C236993 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (aa808-836, FITC) - LS-C236996 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (aa808-836, Biotin) - LS-C237010 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (aa808-836, PE) - LS-C236999 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (aa808-836, Azide-free, HRP) - LS-C248682 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody - LS-C309168 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-KSR2 Antibody (C-Terminus, Azide-free, HRP) - LS-C248681 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-KSR2 Antibody (C-Terminus) - LS-C322574 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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