RNASE3

Gene Symbol	RNASE3
Entrez Gene	6037
Alt Symbol	ECP, RNS3
Species	Human
Gene Type	protein-coding
Description	ribonuclease, RNase A family, 3
Other Description	RNase 3\|cytotoxic ribonuclease\|eosinophil cationic protein\|ribonuclease 3
Swissprots	Q4VBC1 P12724 Q8WZ62 Q9GZN9 Q8WTP7
Accessions	AAG31589 AAG31590 AAG31591 AAG31592 AAG31593 AAG31594 AAG31595 AAG31596 AAL35279 AAL35280 AAL35281 CAA34545 CAA39462 EAW66436 P12724 BC096060 AAH96060 BC096061 AAH96061 BC096062 AAH96062 BX280390 M28128 AAA50283 X15161 CAA33251 NM_002935 NP_002926
Function	Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. {ECO:0000269\|PubMed:19450231, ECO:0000269\|PubMed:2501794}.
Subcellular Location	Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus.
Top Pathways	Asthma

ECP (I-13) - sc-135469 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

ECP (I-13): sc-135469. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

Anti-Ribonuclease 3 antibody - C-terminal - ab181697 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Ribonuclease 3 antibody - ab116017 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

IHC image of Ribonuclease 3 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Ribonuclease 3 antibody - ab135840 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Ribonuclease 3 antibody (ab135840) at 1/500 dilution + 293 cell extracts at 30 µg

Anti-RNASE3 Antibody (aa123-151, APC) - LS-C217206 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151, AP) - LS-C217205 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151, FITC) - LS-C217208 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151, Azide-free, HRP) - LS-C217209 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151, Biotin) - LS-C217207 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151, PE) - LS-C217210 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151) - LS-C324704 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (Val151) - LS-C177247 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Western blot of ECP (V151) pAb in extracts from 293 cells.

Anti-RNASE3 Antibody (aa102-151) - LS-C199836 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Western blot of ECP antibody in extracts from 293 cells.

Anti-RNASE3 Antibody (aa30-154) - LS-C296269 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa34-150) - LS-C296270 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa33-155) - LS-C296271 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 Antibody (aa123-151) - LS-C162637 from LifeSpan Bioscience

Host	Rabbit
Clonality	Polyclonal

Confocal immunofluorescent of RNASE3 Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

RNASE3 Antibody - NBP2-33778 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: RNASE3 Antibody [NBP2-33778] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

RNASE3 Antibody - NBP2-12984 from Novus Biologicals

Host	Rabbit
Clonality	Polyclonal

Western Blot: RNASE3 Antibody [NBP2-12984] - Analysis of RNASE3 (V151) pAb in extracts from 293 cells.

ECP Polyclonal Antibody - bs-8615R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RNASE3 antibody produced in rabbit - HPA056183 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-RNASE3: <B>Cat. No. HPA056183</B>: Immunohistochemical staining of Human bone marrow shows strong cytoplasmic positivity in a subset of hematopoietic cells.

ANTI-RNASE3 (C-TERM) antibody produced in rabbit - SAB1301618 from Sigma-Aldrich

Prices	$326.50
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-RNASE3 (C-term): <B>Cat. No. SAB1301618</B>: RNASE3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 μg/lane).

RNASE3-Antibody-C-term - AP13557b from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	80 µl, 400 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term)(Cat. #AP13557b). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.

RNASE3 Antibody - C-terminal region (OAAB09858) - OAAB09858 from Aviva Systems Biology

Prices	$289.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

ECP Antibody (OAAF05731) - OAAF05731 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Search more