ASPH

Gene Symbol	ASPH
Entrez Gene	444
Alt Symbol	AAH, BAH, CASQ2BP1, FDLAB, HAAH, JCTN, junctin
Species	Human
Gene Type	protein-coding
Description	aspartate beta-hydroxylase
Other Description	A beta H-J-J\|ASP beta-hydroxylase\|aspartyl/asparaginyl beta-hydroxylase\|aspartyl/asparaginyl-beta-hydroxylase\|cardiac junctin\|humbug\|junctate\|peptide-aspartate beta-dioxygenase
Swissprots	Q12797 Q9H291 Q9H2C4 B7ZM95 Q9Y4J0 Q6NXR7 A6NDF4 B4E2K4 A6NHI2 Q9NRI0 E5RGP5 Q9NRI1 Q8TB28 B4DIC9 F5H667
Accessions	CCQ43258 CDM55392 CDM55573 EAW86840 EAW86841 EAW86842 EAW86843 EAW86844 EAW86845 EAW86846 EAW86847 EAW86848 EAW86849 Q12797 AF184241 AAG16983 AF224468 AAF82246 AF224469 AAF82247 AF289489 AAG40811 AF306765 AAG42257 AF339775 AK096383 AK291836 BAF84525 AK295528 BAG58441 AK298577 BAG60769 AK304314 BAG65166 BC015518 AAH15518 BC025236 AAH25236 BC066929 AAH66929 BC142967 AAI42968 BC144362 AAI44363 BC144363 AAI44364 BC166658 AAI66658 BI793083 BM785890 BU623111 BU741737 BX102882 CA420254 CB115846 CB122028 CR996516 DA136318 DN993915 EU718494 ACD93450 FJ461473 ACK37363 HQ447761 ADQ32246 N83868 S83325 AAB50779 U03109 AAA82108 XM_005251235 XP_005251292 XM_005251236 XP_005251293 XM_005251238 XP_005251295 XM_005251239 XP_005251296 XM_005251240 XP_005251297 XM_005251241 XP_005251298 XM_005251242 XP_005251299 XM_005251243 XP_005251300 XM_005251244 XP_005251301 XM_005251246 XP_005251303 XM_005251247 XP_005251304 XM_005251248 XP_005251305 XM_005251249 XP_005251306 XM_005251250 XP_005251307 XM_011517532 XP_011515834 NM_001164750 NP_001158222 NM_001164751 NP_001158223 NM_001164752 NP_001158224 NM_001164753 NP_001158225 NM_001164754 NP_001158226 NM_001164755 NP_001158227 NM_001164756 NP_001158228 NM_004318 NP_004309 NM_020164 NP_064549 NM_032466 NP_115855 NM_032467 NP_115856 NM_032468 NP_115857
Function	Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins. {ECO:0000269\|PubMed:11773073}.
Subcellular Location	Isoform 8: Endoplasmic reticulum membrane; Single-pass type II membrane protein {ECO:0000269\|PubMed:22586105}.
Tissue Specificity	Isoform 1 is detected in all tissues tested. Isoform 8 is mainly expressed in pancreas, heart, brain, kidney and liver. Isoform 8 is expressed in kidney (at protein level). {ECO:0000269\|PubMed:11007777}.

ASPH (A-10) - sc-271391 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

ASPH (A-10): sc-271391. Western blot analysis of ASPH expression in A-10 whole cell lysate.

ASPH (F-7) - sc-365012 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

ASPH (F-7): sc-365012. Western blot analysis of ASPH expression in PC-12 (A) and HeLa (B) whole cell lysates.

ASPH (E-14) - sc-325026 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ASPH (G-20) - sc-33367 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

ASPH (G-20): sc-33367. Western blot analysis of ASPH expression in A-10 (A), C2C12 (B) and L6 (C) whole cell lysates.

ASPH (H-300) - sc-66939 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

ASPH (H-300): sc-66939. Western blot analysis of ASPH expression in HeLa (A), AT-3 (B), PC-12 (C), KNRK (D), c4 (E) and A-10 (F) whole cell lysates.

Anti-Aspartate beta hydroxylase antibody - ab171187 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Aspartate beta hydroxylase antibody (ab171187) at 1/100 dilution + A549 cell line lysate at 35 µg

Anti-Aspartate beta hydroxylase antibody - ab94660 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Aspartate beta hydroxylase antibody (ab94660) at 1 µg/ml + ACHN cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Aspartate beta hydroxylase antibody - ab72846 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-Aspartate beta hydroxylase antibody (ab72846) at 1/500 dilutionLane 1 : Aspartate beta hydroxylase transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-Aspartate beta hydroxylase antibody - ab102587 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Aspartate beta hydroxylase antibody (ab102587) at 1 µg/ml + MCF7 Cell Lysate at 10 µg

Anti-Aspartate beta hydroxylase antibody [EPR10351] - ab172475 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR10351

All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.

Anti-Junctin / ASPH Antibody (aa294-323, AP) - LS-C251886 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa294-323, FITC) - LS-C251888 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa294-323, APC) - LS-C251887 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa294-323, Biotin) - LS-C251889 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (N-Terminus) - LS-C107938 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ASPH antibody LS-C107938 Western blot of Fetal Stomach lysate.

Anti-Junctin / ASPH Antibody (aa294-323, Azide-free, HRP) - LS-C251890 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa287-336) - LS-C115974 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ASPH antibody LS-C115974 Western blot of MCF7 cell lysate.

Anti-Junctin / ASPH Antibody (clone 3G5) - LS-C196703 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3G5

Detection limit for recombinant GST tagged ASPH is approximately 1 ng/ml as a capture antibody.

Anti-Junctin / ASPH Antibody - LS-C309531 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa294-323, PE) - LS-C251891 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody - LS-C309534 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa66-115) - LS-C81641 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ASPH antibody LS-C81641 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-Junctin / ASPH Antibody - LS-C306410 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (HRP) - LS-C317573 from LifeSpan Bioscience

Prices	$305.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Junctin / ASPH Antibody (aa301-331) - LS-C319642 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

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