PSAP

Gene Symbol PSAP
Entrez Gene 5660
Alt Symbol GLBA, SAP1
Species Human
Gene Type protein-coding
Description prosaposin
Other Description proactivator polypeptide|sphingolipid activator protein-1
Swissprots P07292 Q53Y86 P78547 Q92740 P78541 P07602 Q92742 Q92741 Q6IBQ6 P15793 P78546 Q92739 P78558 P78538
Accessions AAC64921 AAG31635 CAA40391 CAA40392 CCQ43188 EAW54436 EAW54437 EAW54438 P07602 AB209776 BAD93013 AK057878 AK129790 AK223290 BAD97010 AK293672 BAG57116 AK299184 BAG61229 AK307721 BC001503 AAH01503 BC004275 AAH04275 BC007612 AAH07612 BC091483 AAH91483 BT006849 AAP35495 CR456746 CAG33027 D00422 BAA00321 DQ587608 DQ893009 ABM83935 DQ896255 ABM87254 J03015 AAB59494 J03077 AAA52560 M12710 M32221 AAA60303 M60255 AAA36594 M60257 AAA36595 M60258 AAA36596 M81355 NM_001042465 NP_001035930 NM_001042466 NP_001035931 NM_002778 NP_002769
Function Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
Subcellular Location Lysosome {ECO:0000269|PubMed:14657016, ECO:0000269|PubMed:21835174}.
Top Pathways Lysosome

saposin (F-15) - sc-100584 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
saposin (F-15): sc-100584. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human spleen tissue showing cytoplasmic localization.

saposin A (A-14) - sc-27008 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

saposin A (S-16) - sc-27007 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

saposin B (E-15) - sc-27014 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
saposin B (E-15): sc-27014. Western blot analysis of saposin B expression in Hep G2 (A), Jurkat (B), U-2 OS (C), A-431 (D), IMR-32 (E) and U-87 MG (F) whole cell lysates.

saposin B (K-12) - sc-27012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
saposin B (K-12): sc-27012. Western blot analysis of saposin B expression in Hep G2 (A), Jurkat (B), U-2 OS (C), A-431 (D), IMR-32 (E) and U-87 MG (F) whole cell lysates.

saposin C (A-3) - sc-374118 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
saposin C (A-3): sc-374118. Western blot analysis of saposin expression in non-transfected 293T: sc-117752 (A), human saposin transfected 293T: sc-170822 (B), A-431 (C), HeLa (D), Hep G2 (E) and NTERA-2 cl.D1 (F) whole cell lysates.

saposin C (B-10) - sc-374119 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
saposin C (B-10): sc-374119. Western blot analysis of saposin expression in non-transfected 293T: sc-117752 (A), human saposin transfected 293T: sc-170822 (B), A-431 (C), HeLa (D), Hep G2 (E) and NTERA-2 cl.D1 (F) whole cell lysates.

saposin C (H-81) - sc-32875 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
saposin C (H-81): sc-32875. Western blot analysis of saposin C expression in MOLT-4 whole cell lysate.

saposin C (L-20) - sc-27018 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PSAP antibody - ab68466 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PSAP antibody (ab68466) at 1/500 dilution + HepG2 whole cell lysate

Anti-PSAP antibody - ab180751 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180751. Blue DAPI for nuclear staining.

Anti-PSAP antibody - ab88251 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PSAP antibody (ab88251) at 1 µg/mlLane 1 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PSAP antibody - ab97441 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PSAP antibody (ab97441) at 1/2000 dilution + Molt-4 whole cell lysate at 30 µg

Anti-PSAP antibody - ab55624 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
PSAP antibody (ab55624) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human spleen tissue.

Anti-PSAP antibody - ab135654 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PSAP antibody (ab135654) at 1/50 dilutionLane 1 : Y-79 cell line lysateLane 2 : A2058 cell line lysateLane 3 : CEM cell line lysateLysates/proteins at 35 µg per lane.

Anti-PSAP antibody - ab81477 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PSAP antibody [4D5F4] - ab189425 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4D5F4
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling PSAP with ab189425 at 5 µg/ml.

Anti-PSAP antibody [4D5F4] - ab166713 from Abcam

Host Mouse
Clonality Monoclonal
Clone 4D5F4
Anti-PSAP antibody [4D5F4] (ab166713) at 1/500 dilution + recombinant Human PSAP protein

Anti-PSAP antibody [EPR10784(B)] - ab166910 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10784(B)
All lanes : Anti-PSAP antibody [EPR10784(B)] (ab166910) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-PSAP antibody [PASE/4LJ] - ab80717 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone PASE/4LJ
Overlay histogram showing HepG2 cells stained with ab80717 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80717, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PSAP / Prosaposin Antibody (Internal) - LS-C145015 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PSAP / Prosaposin Antibody (Internal) - LS-C153545 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PSAP / Prosaposin Antibody (clone 4LJ) - LS-C170843 from LifeSpan Bioscience

Prices $235.00
Sizes 5 ml
Host Mouse
Clonality Monoclonal
Clone 4LJ

Anti-PSAP / Prosaposin Antibody - LS-C170913 from LifeSpan Bioscience

Prices $225.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-PSAP / Prosaposin Antibody (Internal) - LS-C100593 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of SAP Antibody (Center E300) in Y79,A2058,CEM cell line lysates(35 ug/lane). SAP (arrow) was detected using the purified antibody.
Search more