ALDOA

Gene Symbol ALDOA
Entrez Gene 100009055
Alt Symbol -
Species Rabbit
Gene Type protein-coding
Description aldolase A, fructose-bisphosphate
Other Description fructose-bisphosphate aldolase A|muscle-type aldolase
Swissprots P00883 Q28671
Accessions P00883 K02300 AAA31156 XM_008257927 XP_008256149 XM_008257929 XP_008256151 XM_008257930 XP_008256152 NM_001082238 NP_001075707
Function Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. {ECO:0000269|PubMed:17329259}.
Subcellular Location Cytoplasm, myofibril, sarcomere, I band {ECO:0000269|PubMed:15757649}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000269|PubMed:15757649}. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+).
Top Pathways Glycolysis / Gluconeogenesis, Pentose phosphate pathway, Biosynthesis of amino acids, Carbon metabolism, Fructose and mannose metabolism

Anti-Aldolase antibody - ab182915 from Abcam

Host Sheep
Clonality Polyclonal

Anti-Aldolase antibody (Biotin) - ab20683 from Abcam

Prices $359.00
Sizes 1 mg
Host Goat
Clonality Polyclonal

Anti-Aldolase antibody (HRP) - ab20812 from Abcam

Host Goat
Clonality Polyclonal

Anti-Aldolase antibody (HRP) - ab20552 from Abcam

Prices $370.00
Sizes 500 µg
Host Goat
Clonality Polyclonal

Aldolase A Antibody [Biotin] - NB100-2003 from Novus Biologicals

Host Goat
Clonality Polyclonal
Western Blot: Aldolase A Antibody [Biotin] [NB100-2003] - Immunoprecipitation was performed with 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock solution of ~2.5 ug/ml) of purified aldolase in PBS. Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase.

ALDOA antibody - orb34021 from Biorbyt

Prices $187.00, $383.00
Sizes 80 μl (Trial size), 400 μl
Host Rabbit
Clonality Polyclonal
Western blot analysis of anti-ALDOA antibody in mouse liver tissue lysates

Anti-Aldolase Antibody - AC20-0109 from Abcore

Prices $599.00
Sizes 500 µg
Host Sheep
Clonality Polyclonal

Aldolase A, Fructose-Bisphosphate Antibody - abx110931 from Abbexa

Host Rabbit
Clonality Polyclonal

Aldolase A / ALDOA antibody - R1054 from Acris Antibodies

Prices $1,310.00
Sizes 2 ml
Host Goat
Clonality Polyclonal
Immunoprecipitation and Western blot. 300 µl aliquots of whole anti-aldolase antiserum (Cat.-No. R1054) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 µl 0.1 N NaOH.  90 µl of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.  Gel was stained, destained

Anti-ALDOLASE (Rabbit Muscle) (Goat) Antibody - GWB-F0B800 from Genway Biotech

Prices $500.00
Sizes 2 ml
Host Goat

Aldolase Polyclonal Antibody, HRP - PA1-27236 from Thermo Fisher Scientific

Prices $309.00
Sizes 1 mg
Host Goat
Clonality Polyclonal
Immunoprecipitation and Western Blot using Aldolase polyclonal antibody (Product # PA1-27236). 300 µl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase. Precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml). For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated for 24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH. 90 ul of PBS was added, the sample was divided into 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added. The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine g

ALDOA Antibody - CSB-PA001583GA01HU from Cusabio

Host Rabbit

Aldolase antibody (Peroxidase) - orb344354 from Biorbyt

Prices $323.00
Sizes 25 μl
Host Goat
Clonality Polyclonal

Aldolase antibody - orb343922 from Biorbyt

Host Goat
Clonality Polyclonal
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