ALK

Gene Symbol ALK
Entrez Gene 238
Alt Symbol CD246, NBLST3
Species Human
Gene Type protein-coding
Description anaplastic lymphoma receptor tyrosine kinase
Other Description ALK tyrosine kinase receptor|CD246 antigen|mutant anaplastic lymphoma kinase
Swissprots Q9UM73 Q4ZFX9 Q9Y4K6 Q59FI3 Q53RZ4 Q53QQ6
Accessions AAX76514 AAX88892 AAX93126 AAY15027 ACD03579 ACD03580 ACD03581 ACD03582 ACD03583 ACD03584 ACD03585 ACD03586 ACD03587 ACD03588 ACD03589 CAT16962 CCV20015 EAX00506 EAX00507 EAX00508 Q9UM73 AB209477 BAD92714 AK296218 BAG58942 BC156207 AAI56208 BC157090 AAI57091 DA455128 EU788003 ACI47591 EU788004 ACI47592 EU788005 ACI47593 EU788006 ACI47594 EU788007 ACI47595 EU788008 ACI47596 EU788009 ACI47597 EU788010 ACI47598 FJ838786 ACZ57900 FJ838787 ACZ57901 GU128152 ACY79560 GU128153 ACY79561 GU128154 ACY79562 GU128155 ACY79563 GU128156 ACY79564 GU128157 ACY79565 U62540 AAB71619 U66559 AAC51104 NM_004304 NP_004295
Function Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor s
Subcellular Location Cell membrane {ECO:0000269|PubMed:16317043, ECO:0000269|PubMed:9174053}; Single-pass type I membrane protein {ECO:0000269|PubMed:16317043, ECO:0000269|PubMed:9174053}. Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
Tissue Specificity Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. {ECO:0000269|PubMed:9174053}.
Top Pathways Non-small cell lung cancer

PathScan ® Phospho-ALK (Tyr1604) Chemiluminescent Sandwich ELISA Kit - 7020 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Relationship between protein concentrations of lysates prepared using NCI-H2228 cells lysed with (phospho) and without (nonphospho) the addition of phosphatase inhibitors to the lysis buffer. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-ALK (Tyr1586) Sandwich ELISA Kit - 7159 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit - 7324 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Constitutive phosphorylation of NPM-ALK in Karpas299 cells lysed in the presence of phosphatase inhibitors (phospho-lysate) can be detected by PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit #7324. In contrast, only a low level of phospho-NPM-ALK protein is detected in Karpas299 cells lysed without addition of phosphatase inhibitors to the lysis buffer (nonphospho-lysate). However, similar levels of total NPM-ALK protein from either nonphospho or phospho-lysates can be detected by PathScan ® Total ALK Sandwich ELISA Kit #7322. Absorbance at 450 nm is shown in the top figure, while the corresponding Western blots using Phospho-ALK (Tyr1604) Antibody #3341 (right panel) or a total ALK Rabbit mAb #3333 (left panel), are shown in the bottom figure.

PathScan ® Total ALK Chemiluminescent Sandwich ELISA Kit - 7084 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Relationship between protein concentrations of lysates prepared using NCI-H2228 cells lysed with (phospho) and without (nonphospho) the addition of phosphatase inhibitors to the lysis buffer. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan® Total ALK Sandwich ELISA Kit - 7322 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Constitutive phosphorylation of NPM-ALK in Karpas299 cells lysed in the presence of phosphatase inhibitors (phospho-lysate) is detected by PathScan ® Phospho-ALK (Tyr1604) Sandwich ELISA Kit #7324 (top,right). In contrast, only a low level of phospho-NPM-ALK protein is detected in Karpas299 cells lysed without addition of phosphatase inhibitors to the lysis buffer (nonphospho-lysate). However, similar levels of NPM-ALK protein from either nonphospho- or phospho-lysates are detected by PathScan® Total ALK Sandwich ELISA Kit #7322 (top,left). Absorbance at 450 nm is shown in the top figure, while the corresponding western blots using Phospho-ALK (Tyr1604) Antibody #3341 (right) or a Total ALK Rabbit mAb #3337 (left) are shown in the bottom figure. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

Phosphotyrosine ALK / ALK Tyrosine Kinase Receptor ELISA Kit - RAB0934 from Sigma-Aldrich

Category ELISA Kit
Prices $637.00
Sizes 1 kt

Anapastic Lymphoma Kinase, ALK, Human, ELISA Kit - E-EL-H0310 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
Sensitivity 0.094
Range 0.156-10ng/mL

ALK tyrosine kinase receptor, ALK, Human, ELISA Kit - E1113h from ARP American Research Products

Category ELISA Kit
Prices $596.00
Sizes 96 t
Range 0.312-20 ng/mL

Anapastic Lymphoma Kinase, ALK, Rabbit, ELISA Kit - E-EL-RB1513 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Anapastic Lymphoma Kinase, ALK, Chicken, ELISA Kit - E-EL-Ch0384 from ARP American Research Products

Category ELISA Kit
Prices $445.50
Sizes 96 t

Anapastic Lymphoma Kinase, ALK, Porcine, ELISA Kit - E-EL-P0585 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Anapastic Lymphoma Kinase, ALK, Sheep, ELISA Kit - E-EL-S0842 from ARP American Research Products

Category ELISA Kit
Prices $648.00
Sizes 96 t

Anapastic Lymphoma Kinase, ALK, Monkey, ELISA Kit - E-EL-MK0104 from ARP American Research Products

Category ELISA Kit
Prices $585.00
Sizes 96 t

Human ALK ELISA Kit - HA0787 from NeoScientific

Category ELISA Kit
Prices $495.00
Sizes 96 t

Anserine ALK ELISA Kit - AA0787 from NeoScientific

Category ELISA Kit
Prices $495.00
Sizes 96 t

ALK Colorimetric Cell-Based ELISA Kit - CB5032 from Assay Biotechnology

Category ELISA Kit
Prices $380.00, $760.00, $1,140.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

ALK (Phospho-Tyr1507) Colorimetric Cell-Based ELISA Kit - CBP1241 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

ALK (Phospho-Tyr1604) Colorimetric Cell-Based ELISA Kit - CBP1242 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Human ALK (Anapastic Lymphoma Kinase) ELISA Kit - E-EL-H0310 from Elabscience

Category ELISA Kit
Range ~0

ALK, ELISA Kit - MBS2505342 from MyBioSource

Category ELISA Kit
Prices $410.00, $490.00, $2,040.00, $3,590.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Anapastic lymphoma kinase, ELISA Kit - MBS748546 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Anapastic Lymphoma Kinase, ELISA Kit - MBS010510 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Anapastic Lymphoma Kinase, ELISA Kit - MBS097039 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Anapastic Lymphoma Kinase, ELISA Kit - MBS036113 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Anapastic lymphoma kinase, ELISA Kit - MBS753701 from MyBioSource

Category ELISA Kit
Prices $470.00, $675.00, $2,925.00, $5,490.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
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