| Gene Symbol | CHMP4A |
|---|---|
| Entrez Gene | 29082 |
| Alt Symbol | C14orf123, CHMP4, CHMP4B, HSPC134, SHAX2, SNF7, SNF7-1, VPS32-1, VPS32A |
| Species | Human |
| Gene Type | protein-coding |
| Description | charged multivesicular body protein 4A |
| Other Description | SNF7 homolog associated with Alix-2|Snf7 homologue associated with Alix 2|charged multivesicular body protein 4a|chromatin modifying protein 4A|vacuolar protein sorting-associated protein 32-1 |
| Swissprots | Q9P026 Q86SZ8 Q32Q79 Q9BY43 Q96QJ9 Q14D22 |
| Accessions | EAW66065 EAW66066 EAW66067 EAW66068 Q9BY43 AB100262 BAC79376 AF161483 AAF29098 AF212243 AAK14928 AW732226 AY329084 AAQ91193 BC010893 AAH10893 BC107699 AAI07700 BC113533 AAI13534 BC113535 AAI13536 BC143834 BM041056 CB241867 HQ258138 ADR82892 NM_014169 NP_054888 |
| Function | Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, |
| Subcellular Location | Cytoplasmic vesicle membrane. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Note=Membrane-associated. Localizes to large vesicle-like structures. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. |
| Tissue Specificity | Widely expressed. Expressed at higher level in heart, kidney, liver and skeletal muscle. Also expressed in brain, placenta, lung and pancreas. {ECO:0000269|PubMed:14678797}. |
| Top Pathways | Endocytosis |
CHMP4A CRISPR/Cas9 KO Plasmid (h) - sc-402539 from Santa Cruz Biotechnology
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CHMP4A HDR Plasmid (h) - sc-402539-HDR from Santa Cruz Biotechnology
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CHMP4A Double Nickase Plasmid (h) - sc-402539-NIC from Santa Cruz Biotechnology
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CHMP4A Double Nickase Plasmid (h2) - sc-402539-NIC-2 from Santa Cruz Biotechnology
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CHMP4A CRISPR Activation Plasmid (h) - sc-402539-ACT from Santa Cruz Biotechnology
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CHMP4A CRISPR Activation Plasmid (h2) - sc-402539-ACT-2 from Santa Cruz Biotechnology
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CHMP4A Lentiviral Activation Particles (h) - sc-402539-LAC from Santa Cruz Biotechnology
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CHMP4A Lentiviral Activation Particles (h2) - sc-402539-LAC-2 from Santa Cruz Biotechnology
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CHMP4A siRNA (h) - sc-60373 from Santa Cruz Biotechnology
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CHMP4A shRNA Plasmid (h) - sc-60373-SH from Santa Cruz Biotechnology
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CHMP4A shRNA (h) Lentiviral Particles - sc-60373-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU099851 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN6072 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6072 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR01923 from Sigma-Aldrich
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CHMP4A siRNA (Human) - CRH9176 from Cohesion Biosciences
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CHMP4A - MBS8234043 from MyBioSource
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shRNA set against Human CHMP4A (NM_014169.3) - SHH263213 from Creative biogene
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Human CHMP4A siRNA - orb269678 from Biorbyt
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CHMP4A shRNA Plasmid (Human) - CHH9176 from Cohesion Biosciences
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CHMP4A miRNA 3'UTR clone - MiUTR3H-10989 from Creative biogene
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