Ccl2

Gene Symbol Ccl2
Entrez Gene 20296
Alt Symbol AI323594, HC11, JE, MCAF, MCP-1, MCP1, SMC-CF, Scya2, Sigje
Species Mouse
Gene Type protein-coding
Description chemokine (C-C motif) ligand 2
Other Description C-C motif chemokine 2|monocyte chemoattractant protein 1|monocyte chemoattractant protein-1|monocyte chemotactic protein 1|platelet-derived growth factor-inducible protein JE|small inducible cytokine A2|small-inducible cytokine A2
Swissprots Q9QYD7 P10148
Accessions AAA37684 AAA37685 EDL15656 EDL15657 P10148 AF065929 AAF15379 AF065930 AAF15380 AF065931 AAF15381 AF065932 AAF15382 AF065933 AAF15383 AK132590 BAE21250 AK150937 BAE29972 AK151789 BAE30690 AK153443 BAE31998 AK153468 BAE32019 AK153520 BAE32062 BC055070 AAH55070 BC145867 AAI45868 BC145869 AAI45870 CT010187 CAJ18395 NM_011333 NP_035463
Function Chemotactic factor that attracts monocytes, but not neutrophils.
Subcellular Location Secreted.
Top Pathways Chagas disease (American trypanosomiasis), Malaria, Chemokine signaling pathway, Rheumatoid arthritis, NOD-like receptor signaling pathway

MCP-1 Antibody (Mouse Specific) - 2029 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of recombinant mouse MCP-1 protein using MCP-1 Antibody (Mouse Specific).

MCP-1 (FL-148) - sc-28879 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MCP-1 (FL-148): sc-28879. Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.

MCP-1 (M-18) - sc-1784 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of human recombinant MCP-1 (A,C,E) and mouse recombinant MCP-1 (B,D,F). Antibodies tested include MCP-1 (C-17): sc-1304 (A,B), MCP-1 (M-18): sc-1784 (C,D) and MCP-1 (R-17): sc-1785 (E,F).

MCP-1 (R-17) - sc-1785 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of human recombinant MCP-1 (A,C,E) and mouse recombinant MCP-1 (B,D,F). Antibodies tested include MCP-1 (C-17): sc-1304 (A,B), MCP-1 (M-18): sc-1784 (C,D) and MCP-1 (R-17): sc-1785 (E,F).

MCP-1 (ECE.2) - sc-52701 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rat
Clonality Monoclonal

Anti-MCP1 antibody - ab7202 from Abcam

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MCP1 antibody - ab25124 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of MCP1 staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25124, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MCP1 antibody - ab9899 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
staining in colchicine injected mouse brain (including caudate putamen) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.

Anti-MCP1 antibody [4E2/MCP] (Biotin) - ab21397 from Abcam

Prices $372.00
Sizes 100 µg
Clonality Monoclonal
Clone 4E2/MCP

Anti-MCP1 antibody [ECE.2] - ab8101 from Abcam

Prices $384.00
Sizes 50 µg
Host Rat
Clonality Monoclonal
Clone ECE.2
Immunohistochemical analysis of formalin-fixed murine duodenal tissue, staining MCP1 with ab8101.

CCL2/JE/MCP-1 Antibody [Unconjugated] - AF-479-NA from Novus Biologicals

Prices $99.00, $369.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.

CCL2/JE/MCP-1 Antibody [Unconjugated] - AB-479-NA from Novus Biologicals

Prices $359.00
Sizes 1 mg
Host Goat
Clonality Polyclonal

CCL2/MCP1 Antibody (ECE.2) - NBP1-42312 from Novus Biologicals

Prices $229.00
Sizes 200 µl
Host Rat
Clonality Monoclonal
Clone ECE.2

CCL2/MCP1 Antibody (2H5) - NB100-78196 from Novus Biologicals

Prices $329.00
Sizes 500 µg
Host Hamster
Clonality Monoclonal
Clone 2H5
Sandwich ELISA: CCL2/MCP1 Antibody (2H5) [NB100-78196] - The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. NB100-78198)

CCL2/MCP1 Antibody (7H5) - NBP2-36311 from Novus Biologicals

Prices $429.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 7H5

CCL2/JE/MCP-1 Antibody [Biotin] - BAF479 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

CCL2/MCP1 Antibody (RM0172-11K27) - NBP2-12099 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone RM0172-11K27

CCL2/MCP1 Antibody (4E2) - NBP1-43243 from Novus Biologicals

Prices $249.00, $329.00
Sizes 50 µg, 500 µg
Host Hamster
Clonality Monoclonal
Clone 4E2

CCL2/JE/MCP-1 Antibody (123616) [Allophycocyanin] - IC479A from Novus Biologicals

Prices $349.00
Sizes 100 tests
Host Rat
Clonality Monoclonal
Clone 123616
Mouse splenocytes treated with LPS for 48 hours were stained with Rat Anti-Mouse CCL2/JE/MCP-1 APC-conjugated Monoclonal Antibody (Catalog # IC479A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

CCL2/MCP1 Antibody (ECE.2) [Biotin] - NBP1-42280 from Novus Biologicals

Host Rat
Clonality Monoclonal
Clone ECE.2

CCL2/MCP1 Antibody (2H5) [Biotin] - NBP1-43490 from Novus Biologicals

Host Hamster
Clonality Monoclonal
Clone 2H5
Flow Cytometry: MCP1 Antibody (2H5) [Biotin] [NBP1-43490] - Staining of mouse thiogylcolate induced peritoneal exudate cells (PECs) stimulated with LPS in the presence of Brefeldin A for 24 hours. with Anti-mouse F4/80 FITC followed by intracellular staining with Rat IgG2a Isotype Control PE (cat/ 12-4321)(left) or Anti-Mouse CCL2 PE.

CCL2/MCP1 Antibody (2H5) [FITC] - NBP1-43927 from Novus Biologicals

Host Hamster
Clonality Monoclonal
Clone 2H5
Flow Cytometry: MCP1 Antibody (2H5) [FITC] [NBP1-43927] - Staining of 1-day LPS-stimulated human peripheral blood cells with 0.5 ug of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.5 ug of Anti-CCL2 (MCP-1) FITC (filled histogram). Cells in the monocyte gate were used for analysis.

CCL2/MCP1 Antibody (2H5) [PerCP] - NB100-78196PCP from Novus Biologicals

Host Hamster
Clonality Monoclonal
Clone 2H5

CCL2/MCP1 Antibody (2H5) [PE] - NB100-78196PE from Novus Biologicals

Host Hamster
Clonality Monoclonal
Clone 2H5

CCL2/MCP1 Antibody (2H5) [DyLight 350] - NB100-78196UV from Novus Biologicals

Host Hamster
Clonality Monoclonal
Clone 2H5
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