| Gene Symbol | entE |
|---|---|
| Entrez Gene | 947426 |
| Alt Symbol | ECK0587, JW0586 |
| Species | Escherichia coli |
| Gene Type | protein-coding |
| Description | 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex |
| Other Description | - |
| Swissprots | P10378 Q2MBK7 P77773 P15049 |
| Accessions | AAC73695 NP_415126 |
| Function | Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron(2+) atoms. EntE proccesses via a two-step adenylation- ligation reaction (bi-uni-uni-bi ping-pong mechanism). First, it catalyzes the activation of the carboxylate group of 2,3- dihydroxy-benzoate (DHB), via a reversible ATP-dependent pyrophosphate exchange reactions to yield the acyladenylate intermediate 2,3-dihydroxybenzoyl-AMP. It can also transfer AMP to salicylate, 2,4-dihydroxybenzoate, gentisate and 2,3,4- trihydroxybenzoate. In the second step, DHB is transferred from 2,3-dihydroxybenzoyl-AMP onto the phosphopantetheinylated EntB (holo-EntB) to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine. I |
| Subcellular Location | Membrane {ECO:0000269|PubMed:10692387}. |
| Top Pathways | Biosynthesis of siderophore group nonribosomal peptides |
Recombinant Escherichia coli Enterobactin synthase component E(entE) - CSB-YP318739ENV CSB-EP318739ENV CSB-BP318739ENV CSB-MP318739ENV from Cusabio
|
||||||||||
Enterobactin synthase component E (entE), Recombinant Protein - MBS1118774 from MyBioSource
|