| Gene Symbol | entB |
|---|---|
| Entrez Gene | 946178 |
| Alt Symbol | ECK0588, JW0587, entG |
| Species | Escherichia coli |
| Gene Type | protein-coding |
| Description | isochorismatase |
| Other Description | - |
| Swissprots | P0ADI4 P15048 Q2MBK6 |
| Accessions | AAC73696 NP_415127 |
| Function | Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron(2+) atoms. EntB is a bifunctional protein that serves as an isochorismate lyase and an aryl carrier protein (ArCP). Catalyzes the conversion of isochorismate to 2,3-dihydro-2,3- dihydroxybenzoate (2,3-diDHB), the precursor of 2,3- dihydroxybenzoate (DHB). In the enterobactin assembly, EntB functions as an aryl carrier protein phosphopantetheinylated near the C terminus by EntD to yield holo-EntB, which is then acylated by EntE with 2,3-dihydroxybenzoyl-AMP to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine. {ECO:0000269|PubMed:16567620, ECO:0000269|PubMed:16632253, ECO:0000269|PubMed:19699210, ECO:00002 |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:10692387}. |
| Top Pathways | Biosynthesis of siderophore group nonribosomal peptides |
Recombinant Escherichia coli Isochorismatase(entB) - CSB-YP364981ENV CSB-EP364981ENV CSB-BP364981ENV CSB-MP364981ENV from Cusabio
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E.coli entG protein - orb246519 from Biorbyt
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Isochorismatase (entB), Recombinant Protein - MBS1019667 from MyBioSource
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