PDK4
| Gene Symbol | PDK4 |
|---|---|
| Entrez Gene | 5166 |
| Alt Symbol | - |
| Species | Human |
| Gene Type | protein-coding |
| Description | pyruvate dehydrogenase kinase, isozyme 4 |
| Other Description | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial|[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial|pyruvate dehydrogenase kinase, isoenzyme 4|pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial |
| Swissprots | Q16654 |
| Accessions | AAB67048 AAC50670 EAL24128 EAW76757 EAW76758 EAW76759 Q16654 AF334710 AK096428 BAG53287 AK098151 BAG53583 AK223328 BAD97048 AK312424 BAG35334 BC040239 AAH40239 DA923767 DB035430 DQ891256 ABM82182 DQ894441 ABM85367 U54617 AAC50669 NM_002612 NP_002603 |
| Function | Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid |
| Subcellular Location | Mitochondrion matrix. |
| Tissue Specificity | Ubiquitous; highest levels of expression in heart and skeletal muscle. {ECO:0000269|PubMed:14966024, ECO:0000269|PubMed:21816445}. |
PDK4 CRISPR/Cas9 KO Plasmid (h) - sc-401355 from Santa Cruz Biotechnology
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PDK4 HDR Plasmid (h) - sc-401355-HDR from Santa Cruz Biotechnology
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PDK4 Double Nickase Plasmid (h) - sc-401355-NIC from Santa Cruz Biotechnology
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PDK4 Double Nickase Plasmid (h2) - sc-401355-NIC-2 from Santa Cruz Biotechnology
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PDK4 CRISPR Activation Plasmid (h) - sc-401355-ACT from Santa Cruz Biotechnology
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PDK4 CRISPR Activation Plasmid (h2) - sc-401355-ACT-2 from Santa Cruz Biotechnology
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PDK4 Lentiviral Activation Particles (h) - sc-401355-LAC from Santa Cruz Biotechnology
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PDK4 Lentiviral Activation Particles (h2) - sc-401355-LAC-2 from Santa Cruz Biotechnology
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PDK4 siRNA (h) - sc-39030 from Santa Cruz Biotechnology
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PDK4 shRNA Plasmid (h) - sc-39030-SH from Santa Cruz Biotechnology
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PDK4 shRNA (h) Lentiviral Particles - sc-39030-V from Santa Cruz Biotechnology
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PDK4 RNAi - H00005166-R02 from Novus Biologicals
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MISSION® esiRNA - EHU007651 from Sigma-Aldrich
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MISSION® siRNA - SIHK1564 from Sigma-Aldrich
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MISSION® siRNA - SIHK1562 from Sigma-Aldrich
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MISSION® siRNA - SIHK1563 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN16568 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16568 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR11066 from Sigma-Aldrich
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PDK4 siRNA (Human) - CRH3446 from Cohesion Biosciences
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PDK4 CRISPR/Cas9 KO Plasmid (h2) - sc-401355-KO-2 from Santa Cruz Biotechnology
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PDK4 HDR Plasmid (h2) - sc-401355-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human PDK4 (NM_002612.3) - SHH372900 from Creative biogene
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shRNA set against Human PDK4(NM_002612.3) - SHR102372 from Creative biogene
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Human PDK4 siRNA - orb263948 from Biorbyt
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