The concentrations of PAI1-TPA complex were measured in duplicate and interpolated from the PAI1-TPA complex standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1-TPA complex concentration was determined to be 2,040 pg/mL in serum and 2,160 pg/mL in plasma (heparin).

Serum from 10 apparently healthy male donors was measured in duplicate. The mean PAI1-TPA complex concentration was determined to be 4,126 pg/mL with a range of 2,764- 5,584 pg/mL in male donors.

Recombinant proteins for PAI1, TPA, and PAI1-TPA complex were tested in PAI1 SimpleStep ELISA, TPA SimpleStep ELISA and PAI1-Complex SimpleStep ELISA to determine specificity of the PAI-TPA complex ELISA. Background-subtracted data values are graphed. The PAI1-TPA Complex assay does not detect free PAI1 or TPA protein.

PAI1-TPA complex, PAI1 protein and TPA protein were measured in 8-fold diluted serum and plasma. PAI1, TPA, and PAI1-TPA complex values were measured and interpolated from their respective Standard Curve diluted in Sample Diluent NS or Sample Diluent 25BP. Mean +/-SD, n=2, are graphed.