Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-53BP1 Antibody, Affinity Purified:
Cat. No. PLA0077: Detection of Human [deleted Mouse] 53BP1 by Western Blot Sample: Whole cell lysate (20 μg/lane) from U2OS cells resolved on a 3 to 8% tris-acetate gel. Antibody: Affinity purified Rabbit Anti-53BP1 (
Cat. No. PLA0077) used at 0.17 μg/mL. Detection: Chemiluminescence with a 15 second exposure.