Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-LATS2 Antibody, Affinity Purified:
Cat. No. PLA0110: Detection of Human and Mouse LATS2 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-LATS2 Antibody
PLA0110 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
ImmunoprecipitationRabbit Anti-LATS2 Antibody, Affinity Purified:
Cat. No. PLA0110: Detection of Human LATS2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-LATS2 antibody
Cat. No. PLA0110 used for IP at 6 μg/mg lysate. LATS2 was also immunoprecipitated by a LATS2 Antibody B. For blotting immunoprecipitated LATS2,
PLA0100 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.