Anti-CNPase antibody [11-5B]
| Name | Anti-CNPase antibody [11-5B] |
|---|---|
| Supplier | Abcam |
| Catalog | ab80789 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 11-5B |
| Applications | IHC-P WB ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Sheep, Rabbit, Goat, Bovine, Cat, Dog, Human, Pig, Monkey |
| Antigen | Full length native CNPase protein (purified) (Human) |
| Description | Mouse Monoclonal |
| Gene | CNP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing SHSY-5Y cells stained with ab80789 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80789, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/147_ab80789-3-ab80789FC.jpg)
Overlay histogram showing SHSY-5Y cells stained with ab80789 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80789, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Formalin-fixed, paraffin-embedded rat brain stained with ab80789 at 2-4µg/ml using peroxidase-conjugate and AEC chromogen. Note staining of oligodendrocytes and their processes.
Formalin-fixed, paraffin-embedded human brain stained with ab80789 at 2-4µg/ml using peroxidase-conjugate and DAB chromogen. Note staining of oligodendrocytes and their processes.
Product References
2',3'-cyclic nucleotide 3'-phosphodiesterase, an oligodendrocyte-Schwann cell and - 2',3'-cyclic nucleotide 3'-phosphodiesterase, an oligodendrocyte-Schwann cell and
Sprinkle TJ. Crit Rev Neurobiol. 1989;4(3):235-301.
Monoclonal antibody production to human and bovine 2':3'-cyclic nucleotide - Monoclonal antibody production to human and bovine 2':3'-cyclic nucleotide
Sprinkle TJ, Agee JF, Tippins RB, Chamberlain CR, Faguet GB, De Vries GH. Brain Res. 1987 Nov 24;426(2):349-57.