Anti-CDKN2A/p19ARF antibody [5-C3]
| Name | Anti-CDKN2A/p19ARF antibody [5-C3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab26696 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 5-C3 |
| Applications | WB IP ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse p19ARF |
| Description | Rat Monoclonal |
| Gene | Cdkn2a |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deletedLane 2 : MEF total cell lysateSecondaryanti-rat IgG-HRP](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/27081_ab26696_1.jpg)
All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deletedLane 2 : MEF total cell lysateSecondaryanti-rat IgG-HRP
Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.
Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
Product References
Hypergrowth mTORC1 signals translationally activate the ARF tumor suppressor - Hypergrowth mTORC1 signals translationally activate the ARF tumor suppressor
Miceli AP, Saporita AJ, Weber JD. Mol Cell Biol. 2012 Jan;32(2):348-64.
Mouse tissues that undergo neoplastic progression after K-Ras activation are - Mouse tissues that undergo neoplastic progression after K-Ras activation are
Parikh N, Shuck RL, Nguyen TA, Herron A, Donehower LA. Mol Cancer Res. 2012 Jun;10(6):845-55.
RNA helicase DDX5 is a p53-independent target of ARF that participates in - RNA helicase DDX5 is a p53-independent target of ARF that participates in
Saporita AJ, Chang HC, Winkeler CL, Apicelli AJ, Kladney RD, Wang J, Townsend RR, Michel LS, Weber JD. Cancer Res. 2011 Nov 1;71(21):6708-17.
Loss of p19(Arf) facilitates the angiogenic switch and tumor initiation in a - Loss of p19(Arf) facilitates the angiogenic switch and tumor initiation in a
Ulanet DB, Hanahan D. PLoS One. 2010 Aug 27;5(8):e12454.
Myeloid leukemia-associated nucleophosmin mutants perturb p53-dependent and - Myeloid leukemia-associated nucleophosmin mutants perturb p53-dependent and
den Besten W, Kuo ML, Williams RT, Sherr CJ. Cell Cycle. 2005 Nov;4(11):1593-8. Epub 2005 Nov 14.
Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function. - Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function.
Korgaonkar C, Hagen J, Tompkins V, Frazier AA, Allamargot C, Quelle FW, Quelle DE. Mol Cell Biol. 2005 Feb;25(4):1258-71.
Arf-dependent regulation of Pdgf signaling in perivascular cells in the - Arf-dependent regulation of Pdgf signaling in perivascular cells in the
Silva RL, Thornton JD, Martin AC, Rehg JE, Bertwistle D, Zindy F, Skapek SX. EMBO J. 2005 Aug 3;24(15):2803-14. Epub 2005 Jul 21.
Sumoylation induced by the Arf tumor suppressor: a p53-independent function. - Sumoylation induced by the Arf tumor suppressor: a p53-independent function.
Tago K, Chiocca S, Sherr CJ. Proc Natl Acad Sci U S A. 2005 May 24;102(21):7689-94. Epub 2005 May 16.
N-terminal polyubiquitination and degradation of the Arf tumor suppressor. - N-terminal polyubiquitination and degradation of the Arf tumor suppressor.
Kuo ML, den Besten W, Bertwistle D, Roussel MF, Sherr CJ. Genes Dev. 2004 Aug 1;18(15):1862-74.
Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein. - Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Bertwistle D, Zindy F, Sherr CJ, Roussel MF. Hybrid Hybridomics. 2004 Oct;23(5):293-300.