![Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/250 dilution (unpurified) + Raji cell lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25870_ab134120-239862-ab134120upwb.jpg)
Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/250 dilution (unpurified) + Raji cell lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
![Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25871_ab134120-239863-ab134120pwb.jpg)
Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
![All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)Lane 1 : Raji cell lysateLane 2 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25872_CD80-Primary-antibodies-ab134120-1.jpg)
All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)Lane 1 : Raji cell lysateLane 2 : Ramos cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution