Anti-Cardiac Troponin C antibody [1A2]
| Name | Anti-Cardiac Troponin C antibody [1A2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab8285 |
| Prices | $379.00 |
| Sizes | 200 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 1A2 |
| Applications | IHC-P ELISA ELISA WB |
| Species Reactivities | Human |
| Antigen | Full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | TNNC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab8285 staining Human normal skeletal muscle. Staining is localised to the cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Cardiac muscle ring finger-1 increases susceptibility to heart failure in vivo. - Cardiac muscle ring finger-1 increases susceptibility to heart failure in vivo.
Willis MS, Schisler JC, Li L, Rodriguez JE, Hilliard EG, Charles PC, Patterson C. Circ Res. 2009 Jul 2;105(1):80-8.
Kinetics of cardiac thin-filament activation probed by fluorescence polarization - Kinetics of cardiac thin-filament activation probed by fluorescence polarization
Bell MG, Lankford EB, Gonye GE, Ellis-Davies GC, Martyn DA, Regnier M, Barsotti RJ. Biophys J. 2006 Jan 15;90(2):531-43. Epub 2005 Oct 28.