Phospho-p70 S6 Kinase (T421/S424) Affinity Purified PAb, Rabbit IgG
| Name | Phospho-p70 S6 Kinase (T421/S424) Affinity Purified PAb, Rabbit IgG |
|---|---|
| Supplier | R&D Systems |
| Catalog | AF8965-SP |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | Simple Western WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Phosphopeptide containing human, rat, and mouse p70 S6 Kinase T421/S424 sites |
| Description | Rabbit Polyclonal |
| Gene | RPS6KB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of Human and Mouse Phospho-p70 S6 Kinase (T421/S424) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL of Recombinant Human IGF‑1 (Catalog # 291-G1) for 20 minutes and NIH‑3T3 mouse embryonic fibroblast cell line untreated or treated with 100 ng/mL of Human PDGF (Catalog # 120-HD) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-p70 S6 Kinase (T421/S424) by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 66 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Product References
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