Anti-BPTF / FALZ antibody
| Name | Anti-BPTF / FALZ antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72036 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-P |
| Species Reactivities | Human, Chimpanzee, Orangutan |
| Antigen | Synthetic peptide from a region between residue 1350 and 1400 of human BPTF/ FALZ, NP_872579 |
| Description | Rabbit Polyclonal |
| Gene | BPTF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BPTF / FALZ with ab72036 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-BPTF / FALZ antibody (ab72036) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells. at 50 µgLane 2 : Whole cell lysate from Hela cells. at 15 µgLane 3 : Whole cell lysate from Hela cells. at 5 µg
Immunoprecipitation/ Western Blot of FALZ / BPTF. Lane 1: ab72036 at 3µg/mg whole cell lysate for IP, and 0.1 µg/ml for subsequent WB. Lane 2: Control IgG. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 1 second.
ICC/IF image of ab72036 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72036, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.