ab6323 staining beta COP in Hep G2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/100 in PBST + 1% donkey serum) for 1 hour at 22°C. A DyLight® 488-conjugated donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody. Green - beta COP, Red - Golgi (ab24586, anti-Giantin), Blue - Nucleus (DAPI).See Abreview

Untreated HEK293 cells incubated with the COPB ab6323 in a dilution of 1/100. This picture was kindly submitted as part of the review by Evelien Gellynck.

ab6323 at a 1/1000 dilution staining ~ 105 kDa beta COP in melanoma cell lysate by Western blot.  ab6323 at a 1/1000 dilution staining ~ 105 kDa beta COP in melanoma cell lysate by Western blot