ab87269 at 1/250 dilution staining Bcr in human K562 cells by Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
ab87269 at 1/250 dilution staining Bcr in K562 cells by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
ab87269 staining Bcr in Human B cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.5% Triton X-100 in PBS and blocked with 1% BSA for 90 minutes at 20°C. Samples were incubated with primary antibody (1/50; 1% BSA in PBS) for 2 hours 30 minutes at 20°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibodySee Abreview