
All lanes : Anti-Shh Antibody (C-term)(AW5359) at 1/1000 dilution Lane 1: mouse liver lysates Lane 2: mouse lung lysates Lane 3: mouse stomach lysates Lane 4: rat lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded M. lung section using (Mouse) Shh Antibody (C-term)(Cat#AW5359). AW5359 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.