All lanes : Anti-14-3-3 zeta antibody (ab135823) at 1/100 dilutionLane 1 : MDA-MB-231 cell lysateLane 2 : A2058 cell lysateLane 3 : Mouse cerebellum tissue lysateLane 4 : Mouse brain tissue lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse brain tissue labelling 14-3-3 zeta with ab135823 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of MDA-MB-231 cells labelling 14-3-3 zeta with ab135823 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.