Anti-14-3-3 zeta antibody
| Name | Anti-14-3-3 zeta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51129 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R) |
| Description | Rabbit Polyclonal |
| Gene | YWHAZ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
All lanes : Anti-14-3-3 zeta antibody (ab51129)Lane 1 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with no peptideLane 2 : Extracts from 293 cells treated with Forskolin (40nM, 30min) with immunising peptide
All lanes : Anti-14-3-3 zeta antibody (ab51129) at 1/500 dilutionLane 1 : Human 14-3-3 zeta full length protein (ab85268) at 0.1 µgLane 2 : Human 14-3-3 zeta full length protein (ab85268) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes
ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
A unique four-hub protein cluster associates to glioblastoma progression. - A unique four-hub protein cluster associates to glioblastoma progression.
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Frontal-subcortical protein expression following prenatal exposure to maternal - Frontal-subcortical protein expression following prenatal exposure to maternal
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Broadstock M, Lewinsky R, Jones EL, Mitchelmore C, Howlett DR, Francis PT. J Neural Transm. 2012 Apr;119(4):493-6.
17beta-estradiol prevents reduction of retinal phosphorylated 14-3-3 zeta protein - 17beta-estradiol prevents reduction of retinal phosphorylated 14-3-3 zeta protein
Koseki N, Kitaoka Y, Munemasa Y, Kumai T, Kojima K, Ueno S, Ohtani-Kaneko R. Brain Res. 2012 Jan 18;1433:145-52.
Proteomic analysis reveals late exercise effects on cardiac remodeling following - Proteomic analysis reveals late exercise effects on cardiac remodeling following
Bansal A, Dai Q, Chiao YA, Hakala KW, Zhang JQ, Weintraub ST, Lindsey ML. J Proteomics. 2010 Sep 10;73(10):2041-9.