Anti-PPAP2B (21-35) antibody produced in rabbit
| Name | Anti-PPAP2B (21-35) antibody produced in rabbit |
|---|---|
| Supplier | Sigma-Aldrich |
| Catalog | SAB1100866 |
| Prices | $328.50 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | ICC/IF WB |
| Species Reactivities | Human |
| Antigen | synthetic peptide corresponding to amino acids 21-35 of human PPAP2B |
| Description | Rabbit Polyclonal |
| Gene | PPAP2B |
| Conjugate | unconjugated |
| Supplier Page | Shop |
Product images
ImmunofluorescenceAnti-PPAP2B (21-35) : Cat. No. SAB1100866: Immunofluorescence of HUVEC cells using PPAP2B (21-35) , Cat. No. SAB1100866 (red) at a 1:100 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to [email protected]
ImmunoblottingLysate of HEK-293T cells overexpressing human PPAP2B-FLAG Tag was separated on SDS-PAGE, blotted with Anti- PPAP2B (21-35) (Cat. No. SAB1100866) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Antibody dilution 1:500
3. Antibody dilution 1:500 + PPAP2B immunizing peptide (human,21-35)
4. Antibody dilution 1:1,000
5. Negative control (only secondary antibody)
6. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μ/gL
ChartAnti-PPAP2B (21-35) : Cat. No. SAB1100866: Intensity analysis of Anti-PPAP2B (21-35) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control. Yale HTCB IF procedure used.