ImmunohistochemistryCytoplasmic localization of synemin in human skeletal muscle (endothelial cells in tissue vasculature). Fixed tissue was stained with Anti-Synemin (N-terminal) (antibody dilution 1:100) (
Cat. No. S9075) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunohistochemistryLocalization of synemin in human skeletal muscle (striated tissue). Fixed tissue was stained with 1:500 Anti-Synemin (N-terminal) (
Cat. No. S9075) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingRat skeletal muscle extract (S1 fraction) was separated on SDS-PAGE, blotted with Anti-Synemin (N-terminal) (
Cat. No. S9075), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution: 1:1,000
2. Antibody dilution: 1:1,000 + Synemin immunizing peptide (human, 11-27)