![Western Blot: OTUD5 Antibody [NBP1-49937] - Detection of Human and Mouse DUBA by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-DUBA antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. DUBA was also immunoprecipitated by rabbit anti-DUBA antibody which recognizes a downstream epitope. For blotting immunoprecipitated DUBA was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/6630_OTUD5%2520Antibody-Western%2520Blot-NBP1-49937-img0001.jpg)
Western Blot: OTUD5 Antibody [NBP1-49937] - Detection of Human and Mouse DUBA by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-DUBA antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. DUBA was also immunoprecipitated by rabbit anti-DUBA antibody which recognizes a downstream epitope. For blotting immunoprecipitated DUBA was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).