Western blot analysis of extracts from various cell lines using SYVN1 (D3O2A) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human AMFR protein (hAMFR-Myc/DDK; +) or Myc/DDK-tagged full-length human SYVN1 protein (hSYVN1-Myc/DDK; +), using SYVN1 (D3O2A) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Thapsigargin #12758 (1 μM, 16 hr; +) or Tunicamycin #12819 (5 μg/ml, 16 hr; +), using SYVN1 (D3O2A) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from SH-SY5Y cells, untreated (-) or treated with Tunicamycin #12819 (10ug/mL, 16h; +), using SYVN1 (D3O2A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of SYVN1 from 293T cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or SYVN1 (D3O2A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SYVN1 (D3O2A) Rabbit mAb.
Confocal immunofluorescent analysis of SH-SY5Y cells, untreated (left) or treated with Tunicamycin #12819 (10 μg/mL for 16 hrs; center), and HeLa cells (right) using SYVN1 (D3O2A) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).