![Western Blot: UBA52 Antibody [NB300-263] - Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified human ubiquitin was applied in decreasing amounts from 5 to 0.1 mcg. Antibody: Affinity purified rabbit anti-Ubiquitin antibody NB 300-263 used at 0.4 mcg/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/12270_UBA52-Antibody-Western-Blot-NB300-263-img0004.jpg)
Western Blot: UBA52 Antibody [NB300-263] - Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified human ubiquitin was applied in decreasing amounts from 5 to 0.1 mcg. Antibody: Affinity purified rabbit anti-Ubiquitin antibody NB 300-263 used at 0.4 mcg/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).
![Immunohistochemistry: UBA52 Antibody [NB300-263] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Ubiquitin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/12271_UBA52-Antibody-Immunohistochemistry-NB300-263-img0006.jpg)
Immunohistochemistry: UBA52 Antibody [NB300-263] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Ubiquitin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB