p19ARF Antibody (5-C3-1)

Name	p19ARF Antibody (5-C3-1)
Supplier	Novus Biologicals
Catalog	NB200-174
Prices	$99.00, $299.00
Sizes	25 µl, 100 µl
Host	Rat
Clonality	Monoclonal
Isotype	IgG2b
Clone	5-C3-1
Applications	WB FC ICC/IF IHC IHC-F IHC-P IP
Species Reactivities	Human
Antigen	A synthetic peptide containing amino acids 54-75 of the murine p19ARF. [UniProt# Q64364]
Purity/Format	Protein A purified
Description	Rat Monoclonal
Gene	Cdkn2a
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Western Blot: p19ARF Antibody (5-C3-1) [NB200-174] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-174 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analysed by Western blotting, as input controls.

Immunocytochemistry/Immunofluorescence: p19ARF Antibody (5-C3-1) [NB200-174] - Immunofluorescent detection of p19Arf. Wild type mouse embryo fibroblasts (MEF) at passage 5 (panels 1, 2) and NIH3T3 cells, which have deleted the Arf gene (panels 3, 4) were probed with NB200-17.

Immunohistochemistry: p19ARF Antibody (5-C3-1) [NB200-174] - Immunohistochemical staining of sections from a wild type mouse testis taken at P11 were probed with NB200-174 (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue).

Flow (Intracellular): p19ARF Antibody (5-C3-1) [NB200-174] - An intracellular stain was performed on HEK293 cells with p14ARF (5-C3-1) antibody NB200-174 (blue) and a matched isotype control MAB0061 (orange) [A]. An intracellular stain was also performed on THP-1 (known homozygous deletion of gene, PMID: 19635138) cells to ensure antibody specificity [B]. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody (F0113, R&D Systems). *Note: This antibody recognizes both human (p14ARF) and mouse (p19ARF) versions of the protein.

Western Blot: p19ARF Antibody (5-C3-1) [NB200-174] - Analysis of endogenous p19Arf. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE.