Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - Immunocytochemistry of PINK1 antibody (BC100-494 Lot G). HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP.

Western Blot: PINK1 Antibody [BC100-494] - Analysis of PINK1 in HeLa whole cell lysate with and without treatment of 10 uM CCCP. Image courtesy of an anonymous customer review.

Western Blot: PINK1 Antibody [BC100-494] - Western blot image of PINK1 antibody (BC100-494) in multiple cells lines. Human HeLa (lane 1), Mouse NIH-3T3 (lane 2), L929 (lane 3) and Rat PC12 (lane 4) whole cell protein were separated by SDS-PAGE on a 7.5% polyacrylamide gel. Protein was transferred to PVDF membrane and probed with 2 ug/ml BC100-494 in 1% BSA and detected with an HRP-conjugated anti-rabbit secondary antibody using chemiluminescence. PINK1 was detected at approximately 60 kDa (arrowhead).

Western Blot: PINK1 Antibody [BC100-494] - Whole cell protein from HeLa cells treated without or with valinomycin (1 uM for 24h) as indicated were separated by SDS-PAGE on a 7.5% polyacrylamide gel. Protein was transferred to PVDF membrane and probed with 1.0 ug/ml BC100-494 in 1% BSA and detected with an HRP-conjugated anti-rabbit secondary antibody using chemiluminescence. PINK1 was detected at approximately 60 kDa in the treated sample(arrowhead).

Immunocytochemistry/Immunofluorescence: PINK1 Antibody [BC100-494] - HeLa cells were treated with valinomycin (1 uM for 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% triton X-100 in PBS for 10 min. Cells were incubated with BC100-494 at 20 ug/ml for 1h at room temperature, washed 3x in PBS and incubated with Alexa-Fluor488 anti-rabbit secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using NB100-690 with an anti-mouse DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondrial staining may only be visible after treatment with valinomycin or CCCP.