Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of HLA-DRB1 Antibody in WiDr cell line lysates (35 ug/lane). HLA-DRB1 (arrow) was detected using the purified antibody.
Western blot of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Flow cytometric of widr cells using HLA-DRB1 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.