IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)

Name	IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
Supplier	Cell Signaling Technology
Catalog	4814
Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	L35A5
Applications	WB IP IHC-P ICC/IF FC
Species Reactivities	Human, Mouse, Rat, Monkey, Bovine, Pig, Guinea Pig
Antigen	Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding the amino-terminus of human IκBα.
Description	Mouse Monoclonal
Gene	NFKBIA
Conjugate	Amino-terminal Antigen
Supplier Page	Shop

Product images

Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (lower).

Western blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells, using IkBα (L35A5) Mouse mAb (Amino-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human leiomyoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).

Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).

Confocal immunofluorescent analysis of HeLa cells, untreated (left), or TNF-α-treated (#8902, 10 ng/ml for 15 min, right) using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of HeLa cells, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (blue) compared to a nonspecifc negative control antibody (red).