Western blot analysis of extracts from Hela cells, treated with TNF-α (#2169, 10 ng/ml) for the indicated times, using Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (upper) or IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lower).
Western blot analysis of extracts from HT29 cells (lane 1), protein A alone immunoprecipitation (lane 2), immunoprecipitation with nonspecific mouse IgG2a (lane 3), or immunoprecipitation with IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen) (lane 4). Western blot was performed using IκBα Antibody #9242.