
Anti-ARFGAP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.

Flow cytometry of Jurkat cells, using anti-ARFGAP1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow cytometry of HeLa cells, using anti-ARFGAP1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARFGAP1 antibody, and then analyzed by flow cytometry.