![Overlay histogram showing MCF7 cells stained with ab150266 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150266, 0.1μg/1x106 cells) for 30 min at 22ºC.Isotype control antibody (black line) was mouse IgG1 [ICIG1] FITC (ab91356) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/25017_ab150266-245645-ab150266FC.jpg)
Overlay histogram showing MCF7 cells stained with ab150266 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150266, 0.1μg/1x106 cells) for 30 min at 22ºC.Isotype control antibody (black line) was mouse IgG1 [ICIG1] FITC (ab91356) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.