Anti-YY1 antibody - ChIP Grade
| Name | Anti-YY1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab12132 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP ICC/IF ICC/IF WB EMSA |
| Species Reactivities | Mouse, Rat, Human, Monkey |
| Antigen | Synthetic peptide, corresponding to amino acids 109-123 of Human YY1 |
| Description | Rabbit Polyclonal |
| Gene | YY1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab12132 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12132, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required - Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required
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