Anti-YY1 antibody [EPR4652] - Nuclear Loading Control

Name	Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
Supplier	Abcam
Catalog	ab109237
Prices	$395.00, $791.00
Sizes	100 µl, 250 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR4652
Applications	WB IP IHC-P ICC/IF ICC/IF IHC-F
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human YY1 aa 250-350 (internal sequence)
Description	Rabbit Monoclonal
Gene	YY1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/10000 dilution (purified)Lane 1 : HeLa cell lysateLane 2 : Daudi cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/50000 dilution (purified)Lane 1 : Y79 cell lysateLane 2 : HuT-78 cell lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/2000 dilution (purified)Lane 1 : Mouse heartLane 2 : Rat heartLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/1000 dilution (unpurified)Lane 1 : Daudi cell lysateLane 2 : Y-79 cell lysateLane 3 : HuT-78 cell lysateLysates/proteins at 10 µg per lane.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.

Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.

ab109237 (purified) at 1/30 immunoprecipitating YY1 in Y79 cells. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Product References

An intronic variant associated with systemic lupus erythematosus changes the - An intronic variant associated with systemic lupus erythematosus changes the

Zhao H, Yang W, Qiu R, Li J, Xin Q, Wang X, Feng Y, Shan S, Liu Y, Gong Y, Liu Q. Genes Immun. 2012 Oct;13(7):536-42.