Immunohistochemical analysis of formalin fixed and paraffin embedded Human lung carcinoma labeling Wnt5a with ab174963 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-Wnt5a antibody (ab174963) at 1/100 dilution + HeLa cell lysate at 35 µg
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) labeling Wnt5a with ab174963 at 1/10 dilution.FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.