Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling USP11 with ab109232 at 1/570 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).
![All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : Human testis cell lysateLane 4 : Huma fetal kidney cell lysateLane 5 : LnCaP cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18540_USP11-Primary-antibodies-ab109232-2.JPG)
All lanes : Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : Jurkat cell lysateLane 3 : Human testis cell lysateLane 4 : Huma fetal kidney cell lysateLane 5 : LnCaP cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunofluorescent staining of USP11 in HeLa cells, using ab109232 at a dilution of 1/100.