Anti-TNNI1 antibody [EPR17120]

Name	Anti-TNNI1 antibody [EPR17120]
Supplier	Abcam
Catalog	ab202422
Prices	$99.00, $347.00
Sizes	10 µl, 100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR17120
Applications	IHC-P WB IP
Species Reactivities	Human
Antigen	Recombinant full length protein within Human TNNI1 aa 1 to the C-terminus
Description	Rabbit Monoclonal
Gene	TNNI1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-TNNI1 antibody [EPR17120] (ab202422) at 1/10000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal kidney lysateLane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysateLane 4 : HepG2 (Human liver hepatocellular carcinoma) cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TNNI1 with ab202422 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TNNI1 with ab202422 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. No staining on human kidney tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

TNNI1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab202422 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab202422 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal heart whole cell lysate 10 µg (Input). Lane 2: ab202422 IP in Human fetal heart whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202422 in Human fetal heart whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 second.