Anti-Sumo 2+3 [3H12] antibody - Azide free (ab191492) at 1/1000 dilution + HeLa total cell extractSecondaryAlkaline phosphatase conjugated anti-rat IgG
All lanes : Anti-Sumo 2+3 [3H12] antibody - Azide free (ab191492) at 1/1000 dilutionLane 1 : MCF7 total cell extractLane 2 : NIH 3T3 total cell extractLane 3 : CHO total cell extractSecondaryAnti-rat IgG antibody conjugated with HRP at 1/5000 dilution
Immunofluorescent analysis of Mouse primary neural progenitor cells labeling Sumo 2+3 with ab191492 at 1/100 dilution (left panel). DNA was stained with Hoechst (right panel).
Immunofluorescent foci detection in C-33A cells (4% paraformaldehyde-fixed, 0.25 TritonX-100 permeabilized) labeling Sumo 2+3 with ab191492 at 1/100 dilution followed by donkey anti-rat IgG Alexa 488 conjugate.
Immunohistochemical analysis of the coronal section of E16. mouse cerebral cortex labeling Sumo 2+3 with ab191492 at 1/100 dilution (left panel). DNA was stained with Hoechst (right panel).