Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling SP140 with ab171141 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of Hela cells labeling SP140 with ab171141 at 1/25 dilution. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab171141 (1/25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
Anti-SP140 antibody (ab171141) at 1/100 dilution + HL60 cell line lysate at 35 µg