Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121276 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-SELK antibody (ab121276) at 1/250 dilutionLane 1 : RT 4 lysateLane 2 : U 251 MG lysateLane 3 : Human plasma lysate Lane 4 : Human liver lysateLane 5 : Human tonsil lysatedeveloped using the ECL technique
ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human Salivary gland tissue by Immunohistochemistry.