Anti-Sclerostin antibody
| Name | Anti-Sclerostin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab63097 |
| Prices | $394.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Sheep, Human |
| Antigen | Synthetic peptide corresponding to Human Sclerostin aa 12-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | SOST |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Sclerostin antibody (ab63097) at 1/1000 dilution + HeLa cell lysate at 35 µgSecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilutionLane 1 : Mouse liver tissue lysateLane 2 : Mouse lung tissue lysateLane 3 : Rat kidney tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
All lanes : Anti-Sclerostin antibody (ab63097) at 1/1000 dilutionLane 1 : Human kidney tissue lysateLane 2 : Mouse lung tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
Anti-Sclerostin antibody (ab63097) at 1/500 dilution + Rat bone (tibia) whole tissue lysate at 36 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Sclerostin with ab63097. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
Product References
Sirt1 is a regulator of bone mass and a repressor of Sost encoding for - Sirt1 is a regulator of bone mass and a repressor of Sost encoding for
Cohen-Kfir E, Artsi H, Levin A, Abramowitz E, Bajayo A, Gurt I, Zhong L, D'Urso A, Toiber D, Mostoslavsky R, Dresner-Pollak R. Endocrinology. 2011 Dec;152(12):4514-24.
Micro-RNA-31 controls hair cycle-associated changes in gene expression programs - Micro-RNA-31 controls hair cycle-associated changes in gene expression programs
Mardaryev AN, Ahmed MI, Vlahov NV, Fessing MY, Gill JH, Sharov AA, Botchkareva NV. FASEB J. 2010 Oct;24(10):3869-81.